The time of immobility was also examined 24 h after LPS (1 mg/kg i.p.) injections in P2rx7+/+ mice, i.e., when acute symptoms of the LPS induced illness behavior is restored [41]
Soon after preincubation, 3H-5HT (10 nM) was added, and the incubation was ongoing for 10 min. The uptake was terminated by addition of chilly physiological saline, and by vacuum filtration…