Regular human epidermal keratinocytes (NHEKs Cascade Biologics, Portland, OR) HEK1 cells (HEK001 from ATCC, Manassas, VA) and SCC13 cells (gift from Jonathan Garlick, Tufts College, Boston, MA, [40,forty one]) ended up cultured at 37uC in a humidified CO2 incubator as earlier explained [forty two]
Human prostate carcinoma cells (LNCAP ATCC) were cultured as explained . cos-seven cells (ATCC) were being cultured at 37uC in a five% humidified CO2 incubator in DMEM (4 mM L-glutamine,…