Transfected cells made progeny particles with infectious titers achieving up to 46104 TCID50/ml for JFH1Flag-E2- and 46107 TCID50/ml for Jc1Flag-E2-contaminated Huh7.5 cells (Figure
To research the subcellular localization of core and E2, we made Flag-E2-tagged variants of two infectious HCV clones, the authentic isolate that replicates in cultured hepatoma cells (JFH1), and a…