Early onset of differentiation is usually monitored by marker gene expression employing quantitative reverse transcription PCR (qRT-PCR) on complete RNA isolated from increasing cells
Induced differentiation by a described panel of medication. (A) Impedance profiles evaluating NT2 cells treated with retinoic acid (RA, ten mM), hexamethylene bisacetamide (HMBA, 5 mM), 5-azacytidine (AZA, 1 mM),…