The microsomal pellet was re-suspended in one mL of 50 mM Tris pH seven.5 plus protease inhibitors and pre-cleared with one hundred mL of agarose beads (Sigma) (pre-washed in fifty mM Tris pH seven.5 buffer) by rotating for one hr at 4uC

Ultimately, just one research has proposed that presenilins on their own could perform as passive ER calcium channels . Even with all the evidence linking presenilin functionality to intracellular calcium…