For movement cytometric analyses, EBs had been dissociated overnight in 1 mg/ml collagenase B (Roche) at 37uC
For stream cytometric analyses, EBs were dissociated right away in one mg/ml collagenase B (Roche) at 37uC, followed by incubation in TrypLE (Invitrogen) the subsequent early morning for 10?5 min…