E measured 5 times and the result showed the changes in opacity measurements in the intermediate and higher frequency ranges which represents the distinction in between the membrane composition and cytosolic conductance of the exosomes. Summary/Conclusion: A new class of electrical NMDA Receptor manufacturer impedance measurement method was developed with the capability to characterize and distinguish exosomes based on their exclusive dielectric properties as their biogenesis was subjected to systematic alterations under different culture situations. This method is often additional utilized for classification of exosomes based on their cell of origin and may be evolved as a diagnostic tool for characterizing the pathogenic exosomes. Funding: UC Faculty Development Fund.OF22.Characterization of exosomes-based on their one of a kind dielectric properties by a novel electrical impedance measurement system Yuqian Trk custom synthesis Zhanga, Esam Salemb, Takahisa Nakamurac and Leyla EsfandiardaOF22.A snorkel-tag primarily based method for in vivo isolation of recombinant extracellular vesicles Madhusudhan Reddy Bobbilia, Stefan Vogtb, Severin Muehlederc, Carolina Patriolid, Samir Barbariab, Markus Schossererb, Wolfgang Holnthonee, Heinz Redle, AndrG gensf, Samir El Andaloussig and Johannes Grillarih University of Natural Resources and Life Sciences Vienna (BOKU), Vienna, Austria; bUniversity of All-natural Resources and Life Sciences, Vienna (BOKU), Vienna, Austria; cLudwig Boltzmann Institute for Experimental and Clinical Traumatology, vienna, Austria; dUniversity of Organic Resources and Life Sciences, Vienna (BOKU), vienna, Austria; eLudwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria; fKarolinska Institutet, Division of Laboratory Medicine, Stockholm, Sweden; g Division of Laboratory Medicine, Clinical Analysis Center, Karolinska Institutet, Sweden., Stockholm, Sweden; hChristian Doppler Laboratory on Biotechnology of Skin Aging, University of Organic Resources and Life Sciences, Vienna (BOKU), Vienna, AustriaaUniversity of Cincinnati, Non-hispanic or Latino, USA; bCincinnati Children’s Hospital Healthcare Center, Cincinnati, USA; cCincinnati Children’s Hospiltal Health-related Center, Cincinnati, USA; dUniversity of Cincinnati, Cincinnati, USAIntroduction: Exosomes are composed of a lipid bilayer membrane containing nucleic acids, proteins and lipids within the lumen and their compositions reflect their cell of origin. Hence, when the secreting cells are in abnormal microenvironments, the exosomes undergo the compositional changes. We have created a brand new class of electrical impedance measurement method to non-invasively characterize exosomes primarily based on their exceptional dielectric properties. While, the biophysical properties of exosomes for instance size, density and shape happen to be characterized just before, their dielectric properties haven’t been investigated. Strategies: An electrokinetic-based program has been developed to characterize the dielectric properties of exosomes extracted from human hepatocellular carcinoma (HuH-7) cells under various culture situations. Extracted exosomes were initially trapped with dielectrophoresis and further characterized by their dielectric properties as 0.2Vpp was swept from 1 kHz to 50 MHz. Outcomes: The principle in the impedance measurement was adapted in the Maxwell’s mixing theory applied to analyse the dielectric behaviour of cells. Opacity was defined as the ratio of impedance magnitude at highIntroduction: Extracellular vesicles (EVs) emerged as an importa.