Earing loss.Author Contributions: Conceptualization, S.-Y.K.; methodology, S.-Y.K.; formal analysis, S.-Y.K., S.-M.L., and K.-W.K. writing–original draft preparation, K.-J.C. and S.-Y.K.; writing–review and editing, K.-J.C., S.-Y.K., C.-H.L., K.-W.K., and S.-M.L.; funding acquisition, S.-Y.K. and C.-H.L. All authors have study and agreed towards the published version with the manuscript. Funding: This study was supported by funding from the National Analysis Foundation (NRF) of Korea (NRF-2018R1D1A1B07048092 (Approval date: 1 May well 2018) and 2020R1A2C4002594 (Approval date: 1 March 2020)). The APC was funded by 2020R1A2C4002594. Institutional Assessment Board Statement: The Institutional Animal Care and Use Committee of CHA University (IACUC200025) authorized the performed animal experiments. The situations of animal rearing, drug administration, and sacrifice complied with the regulations of your Institutional Animal Care and Use Committee of CHA University. Informed Consent Statement: Not applicable.Int. J. Mol. Sci. 2021, 22,11 ofData Availability Statement: The data presented in this study are obtainable upon request from the corresponding author. Conflicts of Interest: The authors declare no conflict of interest. The funders had no function in the design in the study; inside the collection, analyses, or interpretation of data; in the writing on the manuscript, or within the choice to publish the results.
Metabolic Engineering Communications 13 (2021) eContents lists readily available at ScienceDirectMetabolic Engineering Communicationsjournal homepage: www.elsevier.com/locate/mecMetabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction from the sesquiterpene valenceneMaximilian Dietsch a, 1, Anna Behle a, 1, Philipp Westhoff b, Ilka M. Axmann a, a bInstitute for Synthetic Microbiology, Division of Biology, Heinrich Heine University D seldorf, D seldorf, Germany Plant Metabolism and Metabolomics Laboratory, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University D seldorf, D-40001, D seldorf, GermanyA R T I C L E I N F OKeywords: Metabolic engineering SIRT6 Storage & Stability Cyanobacteria Synechocystis Valencene SesquiterpeneA B S T R A C TCyanobacteria are exceptionally adaptable, fast-growing, solar-powered cell factories that, like plants, are in a position to convert carbon dioxide into sugar and oxygen and thereby create a big quantity of essential compounds. On account of their exceptional phototrophy-associated physiological properties, i.e. naturally occurring isoprenoid metabolic pathway, they represent a extremely promising platform for terpenoid biosynthesis. Here, we implemented a carefully devised engineering strategy to enhance the biosynthesis of commercially eye-catching plant sequiterpenes, in particular valencene. Sesquiterpenes are a diverse group of bioactive metabolites, primarily produced in greater plants, but with often low concentrations and pricey downstream extraction. Within this function we successfully demonstrate a multi-component engineering approach towards the photosynthetic production of valencene within the cyanobacterium Synechocystis sp. PCC 6803. Very first, we improved the flux towards valencene by markerless genomic deletions of shc and sqs. Secondly, we downregulated the formation of carotenoids, that are αvβ3 Purity & Documentation critical for viability of the cell, applying CRISPRi on crtE. Finally, we intended to boost the spatial proximity on the two enzymes, ispA and CnVS, involved in valencene formation by building an operon construct, at the same time as a fusion protein. Combining t.