Ith a pVSV-G plasmid into GP2-293 packaging cells (Clontech) making use of
Ith a pVSV-G plasmid into GP2-293 packaging cells (Clontech) making use of FuGENE transfection reagent (Promega). Viral supernatants have been collected 72 h post-transfection, centrifuged, and added to 624MEL and…