Icles. We have not too long ago improved the contrast and spatial resolution of SPIRI by pupil function engineering and computational imaging. Solutions: In SPIRI, the interference of light reflected in the sensor surface is modified by the presence of particles creating a distinct signal that reveals the size from the particle that is certainly not otherwise visible below a conventional microscope. Employing this instrument platform, we’ve got demonstrated label-free identification and visualization of different viruses in multiplexed format in complex samples in a disposable cartridge. Recently, our technologies was applied to detection of exosomes and commercialized by Nanoview Biosciences for quantified measurement of exosomes on dry sensor chips. We’re currently focusing onISEV2019 ABSTRACT BOOKvarious in-liquid detection too as further improvement on the technique making use of pupil function engineering. Final results: By acquiring several pictures with a partitioned pupil (resulting in structured illumination) and computational imaging, we’ve demonstrated considerable improvement in visibility of low-index nanoparticles in liquid. Additionally, spatial resolution has been enhanced beyond the diffraction limit approaching one hundred nm inside the visible microscopy. We’ve got developed compact and low-cost sensor chips and microfluidic cartridges permitting for study of biological particles (exosomes and also other extracellular vesicles) straight in the bodily fluids with no labels. Summary/Conclusion: In summary, we have demonstrated enhanced visibility of exosomes in SPIRI using pupil function engineering. Funding: EU-INDEXuse of many recognition events in mixture with signal amplification allows detection of exosomes with high specificity and sensitivity. Summary/Conclusion: Here, we go over the application of proximity assays for sensitive detection of exosomes in physique fluids, to visualize the uptake of exosomes by cells, along with the possible of such strategy to be utilized to far better understand the biology on the exosomes and to identify exosomes as illness biomarkers.OF22.A 96 effectively plate format lipid quantification assay with enhanced sensitivity for standardization of experiments with extracellular vesicles Tamas Visnovitza, Xabier Osteikoetxeab, Barbara W. S arc, Judith Mihalyd, P er Lrincze, Krisztina V. Vukmana, Eszter nes T ha, Anna Koncza, Inna Sz sf, Robert Horv hf, Zoltan Vargag and Edit I Buz c Semmelweis University, Dept. of Genetics, Cell- and Immunobiology, Budapest, Hungary; bAstraZeneca, Macclesfield, UK; cSemmelweis University, Budapest, Hungary; dRCNS HAS, Budapest, Hungary; e Division of Anatomy, Cell and Developmental Biology, E v Lor d University, Budapest, Hungary; fNanobiosensorics Laboratory MTA-EKMFA, Budapest, Hungary; gResearch Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, HungaryaOF22.Proximity assays for detection and characterization of exosomes PAK5 Source Masood Kamali-Moghaddam, Ehsan Manouchehri, 5-HT1 Receptor Inhibitor custom synthesis Alireza Azimi, Qiujin Shen, Radiosa Gallini and Claudia Fredolini Uppsala University, Uppsala, SwedenIntroduction: Exosomes get an increased consideration in standard biology too as in medicine. They may be shown to become involved in a lot of biological processes, and are established to hold great potentials as diagnostic and therapeutic tools. On the other hand, there is certainly an unmet want for new and improved technologies for quantitative and qualitative characterization of exosomes to meet challenges connected to these vesicles, such as low concentrations in body f.