Icles. We’ve recently improved the contrast and spatial resolution of SPIRI by pupil function engineering and computational imaging. Procedures: In SPIRI, the interference of light reflected in the sensor surface is modified by the presence of particles generating a distinct signal that reveals the size from the particle that’s not otherwise Flt-3/CD135 Proteins Purity & Documentation visible under a conventional microscope. Utilizing this instrument platform, we have demonstrated label-free identification and visualization of various viruses in multiplexed format in complex samples inside a disposable cartridge. Recently, our technology was applied to Flk-1/CD309 Proteins custom synthesis detection of exosomes and commercialized by Nanoview Biosciences for quantified measurement of exosomes on dry sensor chips. We are currently focusing onISEV2019 ABSTRACT BOOKvarious in-liquid detection also as further improvement on the technique working with pupil function engineering. Final results: By acquiring several images having a partitioned pupil (resulting in structured illumination) and computational imaging, we’ve demonstrated significant improvement in visibility of low-index nanoparticles in liquid. Moreover, spatial resolution has been enhanced beyond the diffraction limit approaching 100 nm in the visible microscopy. We have developed compact and low-cost sensor chips and microfluidic cartridges enabling for study of biological particles (exosomes and also other extracellular vesicles) straight inside the bodily fluids without having labels. Summary/Conclusion: In summary, we’ve got demonstrated improved visibility of exosomes in SPIRI employing pupil function engineering. Funding: EU-INDEXuse of numerous recognition events in combination with signal amplification allows detection of exosomes with high specificity and sensitivity. Summary/Conclusion: Here, we go over the application of proximity assays for sensitive detection of exosomes in physique fluids, to visualize the uptake of exosomes by cells, along with the prospective of such strategy to be employed to far better fully grasp the biology with the exosomes and to recognize exosomes as disease biomarkers.OF22.A 96 well plate format lipid quantification assay with enhanced sensitivity for standardization of experiments with extracellular vesicles Tamas Visnovitza, Xabier Osteikoetxeab, Barbara W. S arc, Judith Mihalyd, P er Lrincze, Krisztina V. Vukmana, Eszter nes T ha, Anna Koncza, Inna Sz sf, Robert Horv hf, Zoltan Vargag and Edit I Buz c Semmelweis University, Dept. of Genetics, Cell- and Immunobiology, Budapest, Hungary; bAstraZeneca, Macclesfield, UK; cSemmelweis University, Budapest, Hungary; dRCNS HAS, Budapest, Hungary; e Division of Anatomy, Cell and Developmental Biology, E v Lor d University, Budapest, Hungary; fNanobiosensorics Laboratory MTA-EKMFA, Budapest, Hungary; gResearch Centre for All-natural Sciences, Hungarian Academy of Sciences, Budapest, HungaryaOF22.Proximity assays for detection and characterization of exosomes Masood Kamali-Moghaddam, Ehsan Manouchehri, Alireza Azimi, Qiujin Shen, Radiosa Gallini and Claudia Fredolini Uppsala University, Uppsala, SwedenIntroduction: Exosomes get an increased attention in basic biology too as in medicine. They’re shown to become involved in a lot of biological processes, and are verified to hold fantastic potentials as diagnostic and therapeutic tools. Nevertheless, there is an unmet have to have for new and improved technologies for quantitative and qualitative characterization of exosomes to meet challenges related to these vesicles, including low concentrations in physique f.