He average lesion two isogenic strains D122 and D122-P. Pathological tests showed that the typical lesion places caused by D122 were smaller sized than thosecaused by D122-P (Figure 6c), suggesting regions brought on by D122 had been smaller sized than those brought on by D122-P (Figure 6c), suggesting that RsPV5 induced hypovirulence inside the virus-infected strain D122. that RsPV5 induced hypovirulence within the virus-infected strain D122.Viruses 2021, 13, x FOR PEER Critique Viruses 2021, 13,9 of 14 9 ofFigure six. Hypovirulence-associated traits in strain D122 of Rhizoctonai solani AG-1 IA. (a) Colony morphology of strains Figure six. Hypovirulence-associated traits in strain D122 of Rhizoctonai solani AG-1 IA. (a) Colony morphology of strains D122 and D122-P after four days of culture on PDA inside the dark; (b) comparison of typical mycelial growth PDA plates D122 and D122-P after 4 days of culture on PDA inside the dark; (b) comparison of average mycelial growth rate onrate on PDA plates on the D122 and D122-P. The lowercase letters (a and b) on b) around the bars bars in b indicate irrespective of whether the differences in the strains strains D122 and D122-P. The lowercase letters (a and prime oftop of thein b indicate no matter if the variations are are statistically significant (p 0.05); Pathogenicity. The symptoms onon detached rice leaves brought on by strains D122and statistically important (p 0.05); (c) (c) Pathogenicity. The symptoms detached rice leaves caused by strains D122 and D122-P at 28 C for 72 h. D122-P at 28 for 72 h.three.six. RNA-seq Nitrocefin manufacturer Analysis of Rhizoctonia solani AG-1 IA Response to RsRV5 Infection three.6. RNA-seq Analysis of Rhizoctonia solani AG-1 IA Response to RsRV5 Infection To identify genes of Rhizoctonia solani AG-1 IA that play key roles in response to To recognize genes of Rhizoctonia solani AG-1 IA that play essential roles in response to RsRV5 infection, RNA-seq technologies was applied to evaluate the expression of fungal RsRV5 infection, RNA-seq technologies was applied to examine the expression of fungal host genes in isogenic strains D122 and D122-P. Data analysis showed that for samples of strains D122 and D122-P. Data analysis showed that for samples host genes of strains D122 and D122-P, there had been a total ofmillion and and 31 million reads, respecstrains D122 and D122-P, there have been a total of 33 33 million 31 million reads, AZD4625 Biological Activity respectively, tively, of which an typical of 73.88 76.17 reads, respectively, were aligned for the Rhiof which an average of 73.88 and and 76.17 reads, respectively, had been aligned for the Rhizoctonia solani AG-1 IA.thisthis study, used absolute logFC 1 and FDR 0.05 0.05 to zoctonia solani AG-1 IA. In In study, we we utilized absolute logFC 1 and FDR to define define DEGs. In comparison with the gene expression information of RsRV5-infection strain D122, total of DEGs. Compared to the gene expression information of RsRV5-infection strain D122, a a total of 3 genes (AG1IA_06216, AG1IA_06615 and AG1IA_09435) as candidates which exthree genes (AG1IA_06216, AG1IA_06615 and AG1IA_09435) as candidates in in which expression was altered werefound in strain D122-P, with two up-regulated (AG1IA_06216 pression was altered were identified in strain D122-P, with two up-regulated (AG1IA_06216 and AG1IA_06615) and a single down-regulated (AG1IA_09435). Gene AG1IA_09435 was supand AG1IA_06615) and one down-regulated (AG1IA_09435). Gene AG1IA_09435 was posed to encodeencode a sulfotransferase household domain-containing protein. Gene supposed to a sulfotransferase family domain-containing protein. Gene AG1IA_0.