Uantitative evaluation of protein [(A) CREB; (E) DARPP; (K) ERK; (O) GluA] and phosphoprotein [(B) PCREB; (F) Thr and Thr PDARPP; (L) PERK; (P) PGluA] levels normalized to actin (mean optical density SEM).Protein levels had been measured in prenatal saline Sodium laureth CAS treated (PSAL) and prenatalcocaine (PCOC) treated mice administered typical saline (sal), cocaine ( mgkg; coc) or D agonist, SKF ( mgkg; skf) as adults.Information are represented as percentage of PSAL mice treated with saline (PSAL sal).p p .PCOC pretreatment groups vs.PSAL pretreatment groups.p p coc or skf treated mice vs.sal treated mice inside the exact same prenatal therapy.Error bars represent SEM.N micegroup.Frontiers in Psychiatry Kid and Neurodevelopmental PsychiatryDecember Volume Write-up Tropea et al.Altered molecular signaling following prenatal cocaineFIGURE Impact of prenatal cocaine remedy on basal, cocaine and SKF induced protein phosphorylation in the nucleus accumbens of adult mice.Representative immunoblots and quantitative analysis of protein [(A) CREB; (E) DARPP; (K) ERK; (O) GluA] and phosphoprotein [(B) PCREB; (F) Thr and Thr PDARPP; (L) PERK; (P) PGluA] levels normalized to actin (imply optical density SEM).Protein levels were measured in prenatal saline (PSAL)treated and prenatal cocaine (PCOC) treated mice administered normal saline (sal), cocaine ( mgkg; coc) or D agonist, SKF ( mgkg; skf) as adults.Data are represented as percentage of PSAL mice treated with saline (PSAL sal).p p .PCOC pretreatment groups vs.PSAL pretreatment groups.p p coc or skf treated mice vs.sal treated mice within the identical prenatal treatment.Error bars represent SEM.N micegroup.www.frontiersin.orgDecember Volume Report PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21563299 Tropea et al.Altered molecular signaling following prenatal cocaine(Figure F) with a trend toward decrease levels of Thr PDARPP (Figure G).Examination of ERK revealed no adjust in the basal levels of ERK or PERK in PCOC mice (Figures K,L, respectively).Examination of GluA revealed considerably larger levels of basal GluA and PGluA in PCOC mice in comparison to PSAL mice (Figures O,P, respectively).Examination of cocaine or SKF induced modifications in phosphoprotein levels revealed that cocaine or SKF substantially improved PCREB in PSAL and PCOC mice compared to saline treated mice (Figure C, PSAL coc vs.PSAL sal and PCOC coc vs.PCOC sal and Figure D, PSAL skf vs.PSAL sal and PCOC skf vs.PCOC sal, respectively).Moreover, the raise in PCREB observed in PCOC mice was drastically augmented in comparison with PSAL mice (Figure C, PCOC coc vs.PSAL coc; Figure D, PCOC skf vs.PSAL skf).Similarly cocaine or SKF remedy drastically elevated Thr PDARPP levels in PSAL and PCOC mice (Figures G,I, respectively) with drastically augmented levels evident in PCOC mice in comparison to that observed in PSAL mice (Figure G, PCOC coc vs.PSAL coc; Figure I, PCOC skf vs.PSAL skf).Cocaine or SKF remedy significantly decreased Thr PDARPP levels in PSAL mice (Figures H,J, respectively).In PCOC mice, cocaine remedy had no effect on Thr PDARPP levels (Figure H) whereas SKF considerably decreased Thr PDARPP levels (Figure J) to levels that had been substantially reduce than that observed in PSAL mice (Figure J, PCOC skf vs.PSAL skf).Examination of PERK levels revealed that cocaine or SKF therapy substantially enhanced PERK levels in each PSAL and PCOC mice (Figures M,N, respectively) with drastically augmented levels evident in PCOC mice in comparison to PSAL mice (Figure M, PCOC.