C vesicle (black arrowhead. (B) Image displaying a CApyr dendrite (Dend and adjacent MF boutons (mfb) and CApyr dendritic spines (asterisks) within a complex thorny excrescence. (B) Greater magnification of boxed area in B.At this MF MedChemExpress LOXO-101 (sulfate) synapse using a CA dendritic spine,a fivelayered gap junction contact [arrow in (B) boxed area,enlarged as (B)],as well as PSDs (arrowheads) of the closely connected chemical synapse. (B) Larger magnification of boxed location in (B) showing a putative gap junction make contact with (arrow amongst MF bouton and CApyr dendritic spine,adjacent for the PSD of a chemical synapse (arrowhead. (C) Image showing part of a CApyr dendrite thorny excrescence,with quite a few CApyr dendritic spines (asterisks) and adjacent MF boutons (mfb) and MF axons (mfa). (C) Greater magnification of boxed location in (C). MF bouton containing quite a few nm round synaptic vesicles surrounding a CApyr dendritic spine. The synaptic get in touch with includes a fivelayered membrane apposition,identified as a gap junction (arrow,and quite a few PSDs of a chemical synapse (arrowheads). (C) Greater magnification of boxed location in (C) displaying a presumptive gap junction (arrow in between a MF bouton along with a CApyr dendritic spine. Scale bars in all tsTEM and FRIL micrographs are . m,unless otherwise indicated.CAb stratum lucidum from adult rat ventral hippocampus in ca. ultrathin sections (ca. m m in every single section). Five synapses with close membrane appositions that we designated as putative gap junctions had been identified between the glutamatergic MFterminal boutons and branched CApyr dendritic spines within classical thorny excrescences (Figure ; dendritic spines indicated by asterisks). In 4 of these five glutamatergic MF terminals,components of chemical synapses [i.e PSDs opposite clusters ofFrontiers in Neuroanatomywww.frontiersin.orgMay Volume Write-up HamzeiSichani et al.Glutamatergic mixed synapses in hippocampusnm synaptic vesicles at presynaptic electron densities of presumptive active zones (Figures A,B,C,arrowheads point to PSDs)] have been observed in close proximity to putative gap junctions (Figures A,B,C; arrows),thereby defining these glutamatergic synapses as probable mixed synapses. Within the fifth instance (not shown),the presumptive gap junction was on the neck of a spine of a CApyr dendrite,together with the spine surrounded by and enclosed within a MF terminal,also constant with its classification as a glutamatergic mixed synapse on a spine within a thorny excrescence. Within these putative gap junctions,the plasma membranes showed a pentalaminar configuration,with a total thickness of nm,characteristic of gap junctions in several other tissues prepared by equivalent fixation and staining solutions (Staehelin,,also Kosaka Kosaka and Hama Fukuda and Kosaka. Nevertheless,the tiny gap junctions described above lack the “semidense cytoplasmic matrix” discovered in these larger neuronal gap junctions (Sotelo and Korn Kosaka Kosaka and Hama Fukuda and Kosaka. These presumptive gap junctions had profiles ranging from to nm (imply nm) in width,suggesting that they contain only a number of dozen connexons,as compared to the nmdiameter dendrodendritic gap junctions reported in hippocampal interneurons (Kosaka and Hama,,that because of their bigger sizes,we PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24571619 estimate to include to connexons.FRIL Evaluation OF MIXED SYNAPSES IN ADULT RAT HIPPOCAMPUS: PROXIMITY OF CxCONTAINING GAP JUNCTIONS AND GLUTAMATE RECEPTORCONTAINING PSDsIn freezefracture replicas of adult rat hippocampus,neurons,and glia were identified according to es.