Owth of Sertoli cells in a dosedependent manner. Notably, the inhibition
Owth of Sertoli cells in a dosedependent manner. Notably, the inhibition rates of groups D and E treated with p38 MAPK inhibitor SB202190 decreased significantly compared with group B and C without SB202190 treatment (Fig. 2B).Effects of cadmium on antioxidant enzymes activities of piglet Sertoli cellsCadmium is a high metal toxin that affects a variety of cellular BMS-214662 msds events, including proliferation and survival. We first probed whether cadmium had an adverse effect on the proliferation of piglet Sertoli cells. After 24 hours’ incubation of piglet Sertoli cells with different concentrations of cadmium chloride, the effect on their proliferation was assessed by MTT assay. As shown in Fig. 2A, Sertoli cells’ proliferation decreased gradually with the increased concentration of cadmium chloride. Cell growth inhibition was significantly different (p < 0.05) in group B and extremely significantly different (p < 0.01) in groups C, D and E when compared to control group A. Moreover, the results showed that cadmium chlorideMDA is regarded as a major marker of lipid peroxidation in tissue, while SOD and GSH-Px are two important enzymes in the antioxidant defense system. After exposure to cadmium chloride, MDA content, SOD, and GSH-Px activities of Sertoli cells were measured and the data was summarized in Fig. 3. Compared with control group A, an increase of MDA content in group B was observed (Fig. 3A). Notably, the increase of MDA content in groups C, D and E was extremely significant (p < 0.01) compared to control group A. These results indicate that the increase of MDA content in Sertoli cells by cadmium is also dose-dependent, although MDA content in group E was lower than that in group D. In direct contrast, the SOD and GSH-Px activities in group B were found to be significantly decreased when compared to control group A, while a marked decline in SOD and GSH-Px activities was observed (p < 0.01) in groups C, D and PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25768400 E (Fig. 3A and 3B). The decrease levels in SOD and GSH-Px activities were closely associated with the concentration of cadmium chloride. After treatment with cadmium chloride for 24 h, MDA content and GSH-Px activity of hepatocytes were determined. As shown in Fig. 3C, an increase of MDA content in cadmium-treated groups was observed when compared to the control A. In contrast, GSH-Px activities in cadmium chloride-treated groups decreased in comparison with control in a dose-response relationship. These results suggest that cadmium caused changes ofFigure 1 Identification of the isolated piglet Sertoli cells. A: Oil red O staining showed that red lipid droplets (arrows) were presented near the nucleus or at the two poles of cytoplasm of the isolated piglet cells, which confirmed the identity of piglet Sertoli cells. Cell nuclei were counterstained with hematoxylin. B: Immunocytochemistry revealed that the isolated cells were positive for FasL (arrows), further verifying the identity of piglet Sertoli cells. Scale bars in A and B = 10 m.Zhang et al. Reproductive Biology and Endocrinology 2010, 8:97 http://www.rbej.com/content/8/1/Page 5 ofFigure 2 Effect of cadmium chloride and p38 MAPK inhibitor SB202190 on proliferation of piglet Sertoli cells. A: MTT assay showed cadmium chloride caused the proliferation inhibition of piglet Sertoli cells. B: MTT assay revealed that p38 MAPK inhibitor SB202190 alleviated the proliferation inhibition of piglet Sertoli cells caused by cadmium chloride. Compared to control group A, “*” indicated si.