The media utilized and their compositions had been as follows. LB is standard Luria-Bertani broth. DMEM stands for Dulbecco’s modified Eagle medium (Gibco ref. 41965)
As this sort of, PLPdependent aminotransferases are assumed to share a typical enzymatic mechanism for alanine transamination that implies cofactor recycling from a covalently connected internal aldimine with an enzyme's…