The VP2 peptide inhibited the binding of phage VP2 to CHO-K1/VPAC1 cells with an IC50 of approximately thirteen.two nM (Determine four), which was considerably decrease than the IC50 of VIP (Figure 5A)
When the phages have been incubated with the goal CHOK1/VPAC1 cells, the temperature was transformed to 4uC to rigorously choose internalized phages, which signifies a deviation from several other panning…