Primary antibodies utilized consist of VEGF (one:1000, abcam, Cambridge, Uk), GAPDH (1:5000, Santa Cruz Biotechnology, Santa Cruz, CA), and b-Actin (1:1000, Sigma-Aldrich, St. Louis, MO)
Equal quantities of protein (10mg, depending on protein of desire) of hippocampal homogenates have been separated on 42% Bis-Tris Gel (Invitrogen, Carlsbad, CA), transferred to .forty five mmol/ L polyvinylidene…